Scyphozoan sequences

DNA sequencing projects involving the Scyphozoa are in their nascent stages. They already are providing valuable information on phylogenetic relationships and taxonomic boundaries at all levels from Class to Species (e.g. Dawson & Jacobs 2001; Collins 2002), but many taxa and molecular markers remain unexplored. With a taxonomically comprehensive multi-genic phylogenetic analysis of the Scyphozoa as our ultimate goal, here we have summarised the scyphozoan sequence data gathered to date in order to highlight areas in need of research. The summary is part of an ongoing project; please contact the authors prior to using these data for publication. Sources, are listed for sequences that are publically available (e.g. publication, GenBank accession, author if unpublished). Unpublished sequences may be released at the discretion of the authors.

See the list of PCR primers available for scyphozoans and closely related taxa.

 

Key to table:

mitochondrial DNA (mtDNA)
COI - Cytochrome Oxidase c subunit I
*Genetic Barcode*
16S - 16S ribosomal DNA (rDNA)
whole - whole mitochondrial genome
form - number of fragments into which the mtDNA genome is split * size of fragments (Kb), linear (L) or circular (C)

nuclear DNA (nDNA)
18S - 18S rDNA (SSU, Small Sub-Unit)
*Genetic Barcode*
ITS1 - Internal Transcribed Spacer One
5.8S - 5.8S rDNA
ITS2 - Internal Transcribed Spacer Two
28S - 28S rDNA (LSU, Large Sub-Unit)


List of sources appears at the bottom of the page.

 
mitochondrial
nuclear
other
 
COI
16S
whole
form
18S
ITS1
5.8S
ITS2
28S
CORONATAE
Atollidae Atolla chuni    
    gigantea    
    parva    
    russelli    
    vanhoeffeni AF100942    
    wyvillei    
    sp. MND_U MND_U MND_U MND_U MND_Up    
Atorellidae Atorella arcturi    
    japonica    
    octogonos    
    subglobosa    
    vanhoeffeni    
Linuchidae Linantha lunulata    
  Linuche aquila    
    draco    
    unguiculata MND_U MND_U MND_U MND_U MND_Up    
Nausithoidae Nausithoë albatrossi    
    atlantica    
    aurea    
    challengeri    
    clausi    
    eumedusoides    
    globifera    
    hagenbecki    
    limpida    
    maculata    
    marginata    
    picta    
    planulophora    
    punctata MND_U MND_U MND_U MND_U MND_Up    
    racemosa MND_U MND_U MND_U MND_Up    
    rubra AF358095    
    thieli    
    werneri    
  Palephyra antiqua    
    indica    
    pelagica    
  Thecoscyphus zibrowii    
Paraphyllinidae Paraphyllina intermedia    
    ransoni    
    rubra    
Periphyllidae Nauphantopsis diomedeae    
  Pericolpa campana    
    quadrigata    
    tetralina    
  Periphylla periphylla MND_U MND_U MND_U MND_Up    
  Periphyllopsis braueri    
    galatheae    
CUBOMEDUSAE    
Carybdeidae Carukia barnesi AF358107    
  Carybdea alata    
    aurifera    
    marsupialis

1*16
or
4*4
L

AF358106    
    rastonii AF358108    
    sivickisi AF358110    
    stiasnyi    
    xaymacana AF358109    
  Tamoya gargantua    
    haplonema    
  Tripedalia binata    
    cystophora 4*4 L L10829    
Chirodropidae Chirodropus gorilla    
    palmatus    
    sp.    
  Chironex fleckeri AF358104 U65477 U65477 U65477 U65477    
  Chiropsalmus buitendijki    
    quadrigatus MND_U    
    quadrumanus    
    zygonema    
    sp. AF358103    
RHIZOSTOMEAE    
Cassiopeidae Cassiopea andromeda BDCH_U    
    depressa    
    frondosa BDCH_U    
    medusa    
    mertensi    
    ndrosia    
    ornata    
    picta    
    vanderhorsti    
    xamachana BDCH_U    
    sp. 1*17 L    
Cepheidae Cephea cephea MND_U    
    coerulea    
    conifera    
    octostyla    
    spp.    
  Cotylorhiza ambulacrata    
    erythraea    
    pacifica    
    tuberculata    
  Netrostoma coerulescens    
    dumokuroa    
    setouchianum    
    typhlodendrium    
  Polyrhiza vesiculosa    
Mastigiidae Mastigias albipunctatus    
    andersoni    
    gracilis    
    ocellatus    
    pantherinus    
    papua MND_U MND_U MND_U MND_U MND_Up    
    roseus    
    sidereus    
    sp.    
  Mastigietta palmipes    
  Phyllorhiza luzoni    
    pacifica    
    peronlesueuri    
    punctata MND_U    
  Versuriga anadyomene    
  Thysanostoma flagellatum    
    loriferum    
    thysanura MND_U MND_U MND_U MND_Up    
Lychnorhizidae Anomalorhiza shawi    
  Lychnorhiza arubae    
    lucerna    
    malayensis    
  Pseudorhiza aurosa    
    haeckeli                        
Catostylidae Acromitoides purpurus                        
    stiphropterus                        
  Acromitus flagellatus                        
    hardenbergi                        
    maculosus                        
    rabanchatu                        
    tankahkeei                        
  Catostylus cruciatus                        
    mosaicus BDCH_U MND_U MND_U MND_U MND_Up    
    ornatellus    
    ouwensi    
    perezi    
    tagi    
    townsendi    
    tripterus    
    turgescens    
    viridescens    
    sp. AF358100    
  Crambione bartschi    
    cooki    
    mastigophora    
  Crambionella annandalei    
    orsini MND_U    
    stuhlmanni    
  Leptobrachia leptopus    
Lobonematidae Lobonema mayeri    
    smithi    
  Lobonemoides gracilis    
    robustus    
    sewelli    
Rhizostomatidae Eupilema inexpectata    
    scapulare    
  Rhizostoma luteum    
    pulmo    
  Rhopilema esculentum    
    hispidum    
    nomadica    
    rhopalophorum    
    verrilli    
Stomolophidae Stomolophus fritillarius    
    meleagris AF358101    
    nomurai    
SEMAEOSTOMEAE    
Pelagiidae Chrysaora achlyos    
    africana    
    blossevillei    
    colorata AF358098    
    depressa    
    fulgida    
    fuscescens    
    helvola    
    hysoscella MND_U    
    lactea    
    melanaster AF358099    
    pacifica    
    plocamia    
    quinquecirrha    
    spp.    
  Pelagia noctiluca    
  Sanderia malayensis    
Cyaneidae Cyanea annasethe    
    buitendijki    
    capillata MND_U 1*17 L U65481 U65481 U65481 U65481    
    citrea    
    ferruginea    
    fulva    
    lamarcki    
    mjöbergi    
    muellerianthe    
    nozakii    
    postelsi    
    purpurea    
    rosea    
    sp. AF358097    
  Desmonema chierchianum    
    comatum    
    gaudichaudi    
    glaciale    
  Drymonema dalmatinum    
    gorgo  
Ulmaridae Aurelia aurita (BOR) D&J_2001 AF461399
AAU19373
1*18 L AY039208
AAU52972
AAU19541
D&J_2001
AF461406
MND_U
AF461406
MND_U MND_Up   neoscox1 (AY096254, AB037147), scox1A (AY096256), scox2 (AY096258, AB036330), scox3 (AY096259, AB037148), Scox-6 (AB036331, AY096261), Scox-7 (AB036691), Scox-8 (AB036692, AY096263), Scox-9 (AB035803), CnPL10 (AB048845), CnVAS1 (AB048852), GAPDH mRNA (AB044092), 5S RNA (AARNA01, JLARR5S), serine protease 1 mRNA (AF486486), serine protease 2 mRNA (AF486487)
    coerulea  
    colpota    
    labiata (LAB) D&J_2001 AF461401 D&J_2001
AF461408
MND_U
AF461408
MND_U MND_Up    
    limbata D&J_2001 D&J_2001 MND_U MND_U MND_Up    
    limbata (LIM) MND_U AF461403 AF461411 AF461411    
    maldivensis    
    solida    
    spp.    
    sp. 1 (UBI) D&J_2001 AF461398 D&J_2001
AF461405
MND_U
AF461405
MND_U MND_Up    
    sp. 2 D&J_2001 D&J_2001 MND_U MND_U MND_Up    
    sp. 3 D&J_2001 D&J_2001 MND_U MND_U MND_Up    
    sp. 4 D&J_2001 D&J_2001 MND_U MND_U MND_Up    
    sp. 5 (MKL) D&J_2001 SJSS_2002 D&J_2001
AF461409
MND_U
AF461409
MND_U MND_Up    
    sp. 6 D&J_2001 D&J_2001 MND_U MND_U MND_Up    
    sp. 7 (ARAB) AF461402 AF461410 AF461410    
    sp. 8 (MCA) AF461404 AF461412 AF461412    
    sp. 9 (TET) AF461400 AF461407 AF461407    
  Aurosa furcata    
  Deepstaria enigmatica    
    reticulum    
  Discomedusa lobata    
    philippina    
    sp.    
  Diplulmaris antarctica    
    malayensis    
  Floresca parthenia    
  Parumbrosa polylobata    
  Phacellophora camtschatica MND_U AF358096 MND_U MND_U MND_U MND_Up    
  Poralia rufescens    
  Sthenonia albida    
  Stygiomedusa gigantea    
  Tiburonia granrojo AY149900    
  Ulmaris prototypus    
    snelliusi    
STAUROMEDUSAE    
Eleutherocarpidae Haliclystus antarcticus    
    auricula    
    borealis    
    kerguelensis    
    salpinx    
    sanjuanensis AF358102    
    steinegeri    
  Lucernaria australis    
    bathyphila    
    haeckeli    
    infundibulum    
    quadricornis    
    saint-hilarei    
    walteri    
  Stenoscyphus inabai    
  Kishinouyea nagatensis    
  Lucernariopsis campanulata    
    vanhoeffeni    
    sp.    
  Sasakiella cruciformis    
    tsingtaoensis    
  Lipkea ruspoliana    
    stephensoni    
    sturdzi    
Cleistocarpidae Brochiella hexaradiata    
  Craterolophus convolvulus 1*18 L AF099104    
    macrocystis    
  Depastromorpha africana    
  Depastrum cyathiforme    
  Halimocyathus lagena    
    platypus    
  Manania auricularia 1*18 L    
  Thaumatoscyphus distinctus    

Sources:
Andres & Campbell (unpubl.; in GenBank)
Bridge et al. (1992)
Bridge et al. (1995)
Collins (2002)
BDCH_U - Holland, Dawson, Crow, & Hofmann (unpubl.)
D&J_2001 - Dawson & Jacobs (2001)
Ender & Schierwater (2003)
Kwong et al. (unpubl.; in GenBank)
MND_U - Dawson (unpubl.), suffix "p" indicates partial sequence
Matsumoto et al. (2003)
Mochizuki & Fujisawa (unpubl.; in GenBank)
Mochizuki (unpubl.; in GenBank)
Rojas & Doolittle (2002)
Sakaguchi & Takahashi (unpubl.; in GenBank)
Schroth et al. (2002)

Compiled by M.N Dawson.